Three-dimensional scaffold with two-stage siRNA delivery to reconstruct periodontal defects

JI Program: Exploratory

Periodontitis is a chronic infectious disease occurring in periodontal support tissues. Macrophages plays an important role in the initiation and progression of periodontitis. The M1 polarization of macrophages plays a key role in pro-inflammatory response, while M2 polarization of macrophages plays a role in anti-inflammation and tissue regeneration. Studies have shown that selectively silencing PTEN or Akt2 could promote M2 polarization of macrophages. Our study aims to construct a three-dimensional nanofibrous scaffolds with "two-stage" RNA release capacity. The first stage is to controlled release a novel non-viral siRNA transfection vector from PLGA microspheres, and the second stage is to efficiently transfect siRNA into target cells. The "two-stage" siRNA delivery vector on the 3D scaffold is hypothesized to selectively silence both PTEN and Akt2, thereby to regulate macrophages from M1 phenotype to M2 phenotype, modulating local inflammatory microenvironment and promoting the regeneration of periodontal defects.